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POTOMAC

Customizable Modular Flow Cytometer

For many applications on the cutting edge of research, “one-size” most definitely doesn’t “fit all.” Whether your work requires the use of light sources unavailable from other suppliers or flow rates outside of the range normally offered, the need for flexibility and customization is often at odds with the capabilities of fixed-layout systems.

We designed and developed a flexible, modular, and completely customizable solution to address this kind of need. With a modular system architecture and built mainly using off-the-shelf components, the Potomac Modular Flow Cytometer adapts to your requirements. The platform was recently redesigned from the ground up to offer both the highest performance and the most flexibility in customization.

You won’t find anything else like it.

The completely redesigned latest generation of the Potomac.

Some details of the Panama flow cytometry software shipped with the Potomac.

Interior of a first-generation 2-laser, 6-detector Potomac.

A second-generation 2-laser, 7-detector Potomac installed at the National Cancer Institute.

The Potomac architecture can support from 1 to 8 lasers (including externally mounted ones), and from 4 to 20 channels of detection. Long-life solid-state excitation lasers are provided as collinear or spatially separated, and either free-space or fiber-coupled. A dedicated, always-on, remotely controlled microscope monitors sample core stability. All filters and beamsplitters are removable and kinematically mounted for hot swapping without the need for any realignment. The detection channels use compact PMTs or silicon photomultipliers (SiPMs). The entire optomechanical structure is based on cage cube assemblies to simplify alignment, modifications, and upgrades. The fluidics control module consists of quadruple regulation of adjustable sheath and sample pressures for maximum core stream stability and flow rate flexibility. A custom DAQ unit manages data collection and signal processing, and the proprietary Panama software provides instant visualization of all channels and parameter combinations.

Specifications†

Excitation Optics

Standard laser options (up to 8):

  • 375 nm (≤ 70 mW)
  • 405 nm (≤ 500 mW)
  • 488 nm (≤ 200 mW)
  • 532 nm (≤ 400 mW)
  • 561 nm (≤ 200 mW)
  • 591/594 nm (≤ 100 mW)
  • 638/640 nm (≤ 500 mW)
  • 785 nm (≤ 350 mW)

Custom laser options (powers vary 30 – 500 mW):

  • 266, 395, 420, 445, 460, 473, 505, 515, 633, 660, 685, 705, 730, 750, 830, 850 nm

Emission Optics 

Scattering channels:

  • FSC: customizable
  • SSC: 90°, 1.2 NA

Fluorescence channels:

  • up to 18 channels
  • customizable bandpass selections

Detectors:

  • PMTs or silicon photomultipliers (SiPMs)

Fluidics

Dual hydrostatic pressure injection option:

  • 8-L sheath capacity, pressure up to 30 psig
  • Sample injection speed: 0.3 – 150 µL/min

Syringe drive injection option:

  • Sample size: 5, 10, 20, 50, or 100 µL
  • Injection speed: 0.1 – 60 µL/min

Signal Processing

Data formatting:

  • Flow Cytometry Standard (FCS) 3.0
  • CSV files
  • optional: raw waveforms

Performance 

Sensitivity (405-nm excitation, 466/40-nm channel):

  • BV421 ≤ 100 MESF (est.)
  • 8/8 Spherotech Rainbow bead peaks

Nanoparticle detection (375+405-nm excitation, FSC/SSC):

  • 160-nm Megamix-Plus

Resolution (375-nm excitation):

  • FL CV 4% (G1 peak)
  • FSC CV 2% (ThermoFisher W500CA beads)

Throughput:

  • 10,000 events/s (typ.)

Installation Requirements 

Dimensions:

  • 36” x 20” x 23” (W x D x H) (monitors, sheath and waste tanks separate)

Weight:

  • 200 lbs. (2-laser, 7-detector system; monitors, sheath and waste tanks separate)

Environmental:

  • 15°–30°C, 60% RH

Power:

  • North America: 120 VAC, 50/60 Hz, 8A
  • Japan: 100 VAC, 50/60 Hz, 8A
  • Rest of world: 230 VAC, 50/60 Hz, 5A

 

†Specifications subject to change without notice.

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